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KMID : 1134820180470030235
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Journal of the Korean Society of Food Science and Nutrition
2018 Volume.47 No. 3 p.235 ~ p.242
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Inhibitory Effect of Skate Skin Collagen on Hepatic Lipid Accumulation through Regulation of Lipid Metabolism
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Lee Hyun-Jung
Woo Min-Ji
Song Yeong-Ok
Noh Jeong-Sook
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Abstract
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Skate is a benthic animal found in the deep sea. Skate byproducts (skin, cartilage, and bone) are generated in large amounts and disposed of as waste during processing. Skate skin contains a high level of collagen, but few studies have investigated the health benefits of collagen beyond those on skin function. Therefore, this study was conducted to investigate the effects of collagen derived from skate skin on lipid metabolism in the liver using an obese animal model. To accomplish this, mice were divided into three experimental groups, Normal (m/m mice), Control (vehicle-treated db/db mice), and Collagen (200 mg/kg body weight collagen-treated db/db mice). Distilled water as the vehicle or collagen was orally administered to mice daily for eight weeks. Plasma and hepatic lipid levels were examined and the expression of hepatic proteins related to lipid metabolism were evaluated by western blotting analysis. Plasma and hepatic triglyceride and total cholesterol levels were significantly lower in the Collagen group (P<0.05), but were similar to those in the Normal group when compared to the Control. Additionally, expression of hepatic proteins associated with fatty acid synthesis (sterol regulatory element-binding proteins-1, fatty acid synthase, and acetyl-CoA carboxylase α) were decreased in the Collagen group, whereas phospho-activated protein kinase and beta-oxidation-related protein (peroxisome proliferator-activated receptor α and acetyl-CoA oxidase 1) were increased (P<0.05). Moreover, expression of proteins involved in cholesterol synthesis (sterol regulatory element-binding proteins-2) were decreased in collagen-treated mice, but those of the cytochrome P450 family 7 subfamily A member 1, which are involved in cholesterol export, increased (P<0.05). Our findings indicate that collagen derived from skate skin effectively suppressed hepatic lipid accumulation through down-regulation of fatty acid and cholesterol synthesis and up-regulation of beta-oxidation and cholesterol export.
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KEYWORD
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skate, collagen, fatty acid synthesis, cholesterol synthesis, fatty acid oxidation
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